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TABLE OF CONTENTS SECTION 1. SECTION 2. SECTION 3. SECTION 4. SECTION 5. SECTION 6. INTRODUCTION .C-2 GETTING STARTED.C-4 FOOD TABLE .C-6 MEAL PLANNING.C-7 FAST FOOD TRAPS .C-9 ESSENTIAL ITEMS TO REMEMBER .C-10. Response Table 2 ; was found for ICP4 pools 3 4 and 5 6 when the 10X dose of peptides was used. Intracellular cytokine staining assayed by Flow cytometry using the same peptide pools, and run in parallel with the ELISPOT assay, confirmed that the responses measured were from the CD8 population data not shown ; . One feature of CD4 ELISPOTs is that they are typically very large Figure 1A ; and can easily be seen without a microscope. The CD8 cell population in the IFN- ELISPOT assay was not responsive to the 1X dose of peptide Figure 1B ; and required the higher 10X dose for stimulation Figure 1C ; . CD8 ELISPOTs were typically smaller than the CD4 ELISPOTs and ketorolac.
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