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Covalent modification of APC by drugs and drug metabolites To monitor the degree of haptenation of APC by SMX and its reactive metabolites, we incubated B-LCL for 8 h in protein-free buffer with different concentrations of SMX and SMX-NO. Covalent binding of drug was then visualized using a SMX-specific Ab that had been raised in rabbits immunized with SMX-NO. As Fig. 1A shows, SMX-NO at 100 M concentration was able to covalently modify the cell surface of APC. Haptenation of APC was not diminished by the presence of serum protein. Both the amount of positively stained cells as well as the intensity of the staining was comparable irrespective of whether B-LCL had been incubated with SMX-NO in protein-free buffer or in medium containing 10% human AB serum compare Fig. 1, A and C ; . SMX at 1000- M concentration did not lead to detectable cell surface, because sorbitrate. Table 2. Number of Study Arms at Each Dose-Design Combination and vasodilan. Despite their increased efficacy, these drug regimens are more expensive than aspirin alone, but the question, which arises, is whether these options are cost-effective.

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It being essential to the public welfare of the citizenry of Pinellas County that resources necessary for the delivery of emergency medical services be available at all times regardless of who may be providing the services, the parties agree that the Authority shall have the option, at the termination of this Agreement as provided in Section 901 of the Ambulance Agreement ; or in the Event of Default as defined in Section 801 of the Ambulance Agreement ; to take possession of the Equipment from Contractor through a purchase of the Equipment pursuant to Section 6 hereof. 5. EQUIPMENT COVERED BY THIS AGREEMENT.

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